功能性食品中生物活性肽(BAPs):从生产与验证到商业化、监管壁垒及未来前景 - 生物通
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¡¶JOURNAL OF FOOD SCIENCE¡·£ºBioactive Peptides in Functional Foods: From Production and Validation to Commercialization, Regulatory Hurdles, and Future Prospects
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1 Introduction
ÉúÎï»îÐÔëÄ£¨BAPs£©ÊÇÇ×±¾µ°°×ÖÐÎÞ»îÐÔ¡¢Êͷźó²úÉúÉúÀíЧӦµÄ2¨C20¸ö°±»ùËᣨAA£©Æ¬¶Î£¬À´Ô´º¸ÇÈé¡¢Èâ¡¢µ°¡¢´ó¶¹¡¢¹ÈÎï¡¢¶¹À༰ÓãÔå¡£Æä½á¹¹¨C»îÐÔ¹ØÏµÓÉAA×é³É¡¢ÐòÁС¢µçºÉ¡¢ÊèË®ÐÔºÍÁ½Ç×ÐԵȾö¶¨£¬¹¦ÄܰüÀ¨¿¹¸ßѪѹ¡¢¿¹Ñõ»¯¡¢¿¹¾ú¡¢ÃâÒßµ÷½Ú¡¢¿¹ÌÇÄò²¡µÈ¡£ValorizationÍÆ¶¯¸±²úÎïת»¯Îª¸ßÖµÅäÁÏ¡£±¾×ÛÊöÆÀ¹À´ÓÉú²ú¡¢ÑéÖ¤µ½ÉÌÒµ»¯¡¢¼à¹ÜºÍǰ¾°¡£
2 The Discovery and Validation of BAPs: A Critical Assessment
2.1 Generation of Bioactive Peptides
2.1.1 Conventional Approaches
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2.1.2 Emerging Production Technologies and Their Industrial Potential
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2.2 Isolation, Purification, and Characterization
2.2.1 Advanced Separation Methodologies
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2.2.2 Peptide Identification and Sequencing Techniques
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2.3 A Multi-Tiered Approach to Bioactivity Validation
2.3.1 In Silico Prediction
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2.3.2 In Vitro Screening
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2.3.3 Cellular and In Vivo Models
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2.4 Mechanistic Basis of Major Biological Activities
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3 BAP Commercialization
3.1 Technical Hurdles in Product Formulation
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3.1.1 Oral Absorption, Systemic Stability, and Tissue Distribution
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3.1.2 Sensory and Matrix Constraints
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3.2 Innovative Formulation and Delivery Strategies
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3.2.1 Comparative Nano- and Micro-Delivery Platforms
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3.2.2 Allergenicity and Safety Considerations of BAPs
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3.3 Manufacturing Scale-Up, Quality Control, and Industrial Implementation
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3.4 Case Studies of Commercial Products
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4 Navigating Regulatory Landscape and Health Claims
4.1 The Global Regulatory Landscape: An Overview
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4.2 FDA (Us) vs. EFSA (Eu)
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4.3 Regulatory Pathways in Asia-Pacific Markets
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4.4 Key Hurdles to Regulatory Approval
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5 Future Prospects and Research Gaps
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6 Conclusions
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