Fabrication and characterization of biocompatible BPC-157 based chitosan hydrogel - Springer Nature Link
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Abstract
Multifunctional hydrogels are essential for advanced biomedical applications. In this study, CH/BPC composite hydrogel was fabricated and characterized for their physicochemical properties. The formulation exhibited a porous structure, analyzed through scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Fourier-transform infrared spectroscopy (FTIR) showed the presence of hydrogen bond between BPC-157 peptide and chitosan backbone, indicating successful peptide incorporation within the hydrogel matrix. The composite hydrogel exhibited a balanced water vapor transmission rate (2,270 ± 35 g/m2 /day), along with remarkable properties of injectability, self-healing capability, and adhesiveness. Furthermore, the hydrogel demonstrated excellent encapsulation (98.9 ± 0.8%) of BPC-157, with release profile of 81.2 ± 2.9% within 24 h. In addition, the CH/BPC hydrogel demonstrated potent antibacterial efficacy, achieving up to 45.9% (Escherichia coli) and 65.0% (Staphylococcus aureus) inhibition. The fabricated hydrogel also showed a hemolysis rate of less than 5%, indicating acceptable hemocompatibility. Collectively, these findings indicate that CH/BPC hydrogel possesses desirable physicochemical and antibacterial characteristics, highlighting their potential as multifunctional hydrogel for future biomedical applications.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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A. wrote the original draft and was responsible for formal analysis, investigation, and methodology. M.D. and M.S. contributed to the conceptualization of the study. S. performed re-analysis of the data in the revised manuscript. R.K. was responsible for editing. S.G. contributed to the analysis. S.B. was responsible for data curation. All authors read and approved the final manuscript.
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All animal-related procedures were conducted in accordance with the institutional ethical guidelines for the care and use of laboratory animals, following approval from the Institutional Animal Ethics Committee (IAEC) of Banasthali Vidyapith, Rajasthan, under approval number BV/IAEC/September2023/10.
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Arunim, Dagar, M., Solanki, M. et al. Fabrication and characterization of biocompatible BPC-157 based chitosan hydrogel. 3 Biotech 16, 312 (2026). https://doi.org/10.1007/s13205-026-04951-4
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- DOI: https://doi.org/10.1007/s13205-026-04951-4